IN VITRO COMPARISON OF MINIMAL INHIBITORY CONCENTRATION (MIC) GRISEOFULVIN, ITRACONAZOLE, AND TERBINAFINE AGAINST THE CAUSATIVE AGENT OF DERMATOPHYTOSIS ON GLABROUS SKIN IN MAKASSAR

Sari Handayani; Zakiani Sakka; Safruddin Amin; Nasrum Massi

doi:10.31970/ma.v7i01.299

Sari Handayani Fakultas Kedokteran Universitas Alkhairaat
Zakiani Sakka Departemen Kulit dan Kelamin, Fakultas Kedokteran, Universitas Alhairaat Palu, Sulawesi Tengah
Safruddin Amin Departemen Ilmu Kesehatan Kulit dan kelamin Fakultas Kedokteran Universitas Hasanuddin Makassar
Nasrum Massi 2Departemen Ilmu Kesehatan Kulit dan kelamin Fakultas Kedokteran Universitas Hasanuddin Makassar

DOI: https://doi.org/10.31970/ma.v7i01.299

Keywords: dermatofitosis, griseofulvin, itrakonazole, KHM, terbinafin

Abstract

ABSTRAK

Dermatofitosis adalah infeksi jamur golongan dermatofit, yaitu organisme yang menyerang jaringan keratin pejamunya. Tiga genus dermatofit yaitu: Epidermophyton, Microsporum dan Trichophyton.Menentukan KHM griseofulvin, itrakonazol dan terbinafin terhadap isolat agen penyebab dermatofitosis kulit glabrous. Pengambilan data deskriptif potong lintang dengan uji kepekaan griseofulvin, itrakonazol dan terbinafin terhadap isolat koloni dermatofit yang tumbuh melalui tehnik mikrodilusi kaldu pada Laboratorium Mikrobiologi RS Pendidikan UNHAS Makassar. Griseofulvin, itrakonazol dan terbinafin peka terhadap sebagian besar agen penyebab dermatofitosis pada kulit glabrous di Makassar yaitu dengan persentase kepekaan griseofulvin 96,15%, itrakonazole 96,30% dan terbinafin 100% dari 27 isolat dermatofit yang dapat diidentifikasi. Spesies yang telah resisten terhadap griseofulvin adalah Trichophyton rubrum, spesies yang resisten terhadap itrakonazole adalah Microsporum audouinii sedangkan terbinafin peka terhadap keduaSpesies Trichophyton dan Microsporum.KHM Itrakonazole lebih rendah dari griseofulvin dan lebih tinggi dibanding Terbinafin mengindikasikan bahwa terbinafin merupakan antifungal yang lebih peka dibanding kedua golongan obat tersebut.

ABSTRACT

Dermatophytosis is a fungal infection of the dermatophyte group, namely organisms that attack the keratin tissue of their host. The three genera of dermatophytes are: Epidermophyton, Microsporum and Trichophyton. Objectives: Determine the MIC of griseofulvin, itraconazole and terbinafine against isolates of the causative agent of glabrous skin dermatophytosis. Methods: Collecting descriptive cross-sectional data using griseofulvin, itraconazole and terbinafine sensitivity tests on dermatophyte colony isolates growing using the broth microdilution technique at the Microbiology Laboratory of the Makassar UNHAS Teaching Hospital. Result: Griseofulvin, itraconazole and terbinafine were sensitive to most of the agents that cause dermatophytosis on glabrous skin in Makassar, namely with a sensitivity percentage of griseofulvin 96.15%, itraconazole 96.30% and terbinafine 100% of the 27 dermatophyte isolates that could be identified. The species that is resistant to griseofulvin is Trichophyton rubrum, the species that is resistant to itraconazole is Microsporum audouinii while terbinafine is sensitive to both Trichophyton and Microsporum species. Conclusions: The MIC of Itraconazole is lower than griseofulvin and higher than Terbinafine, indicating that terbinafine is a more sensitive antifungal than these two groups of drugs.

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References

Rinaldi MG. Dermatophytosis: epidemiological and microbiological update. J Am Acad Dermatol; 2000; 43: S120-4.

Chen SC, Sorrel TC. New drugs, Old drugs: Antifungal agents. MJA, 2007; 187: 404-9.

Roberts B, Friedlander S. Tinea capitis : a treatment update. Pediatr Ann. 2005; 34: 191-200.

Arrese JE, Pierard GE. Tre,atment Failures and Relapses in Onychomycosis: A Stubborn Clinical Problem. Dermatology. 2003; 207: 255-60.

Tripathi KD. Anti fungal drugs. In: Essentials of Medical Pharmacology. 1999; 4: 770-8.

Winnington P. Analisis of Dermatophyte Species Isolated in the British Isles between 1980 and 2005 and review of worldwide dermatophyte trends over the last three decades. Med Mycol. 2008; 12: 54-8.

Niewerth M, Splanemann V, Korting H, Ring J, Abeck D. Antimicrobial susceptibility sting of dermatophytes- comparison of the agar macrodilution and broth microdilution tests. Chemotherapy. 1997; 44: 31-5.

Hazen, KC. Evaluation of in vitro susceptibility of dermatophytes to oral antifungal agents. J Am Acad Dermatol. 2000; 43: S125-9.

Rassai S, Feily A, Sina A, Derakhshanmehr F. Some Epidemiological Aspects Of Dermatophyte Infections In Southwest Iran. Acta Dermatovenerol Croat. 2011; 19(1):13-5.

Ameen M. Epidemiology of superficial fungal infections. Clin in Dermatol. 2010; 28: 197-201.

Alio A, Mendoza M, Zambrano A, Al E. Dermatophytes growth curve and in vitro susceptibility test: a broth micro-titration method. Med Mycol. 2005; 319-25.

Goh C, Tay Y, Ali K, Koh M, Seow C. In vitro evaluation of griseofulvin, ketoconazoles and itraconazoles against various dermatophytes in singapore. Int J Dermatol. 1994; 33: 733-7.

Hazen KC. Fungicidal versus fungistatic activity of terbinafine and itraconazole: an in vitro comparison. J Am Acad Dermatol. 1998; 38: S37-41.

Elewski BE, Caceres HW, DeLeon L, Shimy SE, Hunter JA, Korotkiy N, et al. Terbinafine hydrochloride oral granules versus oral griseofulvin suspension in children with tinea capitis: results of two randomized, investigator - blinded, multicenter, international, controlled trials. J Am Acad Dermato. 2008; 59: 41-54.

Leyden JJ. Pharmacokinetics and pharmacology of terbinafine and itraconazole. Journal of the American Academy of Dermatology. 1998; 38: 5.

Tey HL, Tan ASL, Chan YC. Meta-analysis of randimized, controlled trials comparing griseofulvin and terbinafine in the treatment of tinea capitis. J Am Acad Dermatol. 2011; 64: 663-70.